Safe Microbiological Techniques (VMT)

1. All ML-I related work can only be performed by those people that have permission from the Biological Safety Officer (BVF). Work according to the rules, even if you believe there is no apparent risk.

2. During ML-I related work all doors and windows have to be closed. Verify that insects and other pests are not present in the lab.

3. Wear a closed laboratory coat. Do not take this labcoat outside the ML-I area unless it is absolutely necessary for the experiment. In case of a contamination of the labcoat, sterilize the labcoat first, with bleach or by autoclaving, before washing.

4. Clean and sterilize spills immediately. Report serious contamination immediately to the BVF.

5. It is absolutely prohibited to eat, drink or smoke, or to have cups, plates, mugs or silverware in the ML-I area.

6. Pipetting by mouth is prohibited. Used pipets are collected in a disinfecting solution.

7. Prevent aerosols. These may be created by -splashing drops; -pouring of liquids; - discharging pipets; -opening of moist plugs; - using inoculating loops that are too hot. Use needles only if there is absolutely no alternative.

8. Glassware and instruments that have been in contact with GMO's (Genetically Modified Organisms, GGO in Dutch) have to be sterilized or disinfected before being washed, reused or discarded. Biological waste has to be collected in autoclavable plastic bags, which are autoclaved before discarding (use indicator tape to demonstrate that the bag was autoclaved).

9. Wash hands with soap and water after work and before leaving the room. Bench surface areas have to be cleaned and disinfected daily. Keep area clean and organized.

10. Record the general nature of the work clearly in a lab journal.