Instructions for using GMO’s in the isotope laboratory




* Uptake studies in whole cells
* Metabolic labelling of cells

Uptake studies in whole cells

- transport GMO’s in secondary containment to the isotope laboratory- in the isotope laboratory treat the GMO’s under the appropriate containment regime- distribute the GMO’s in disposable tubes for the experiments- the following waste streams are discriminated- decontaminate any set-up, like the uptake apparatus, with 70% ethanol after use.
o GMO’s that end up in scintillation liquid may be considered ‘inactivated’ and the waste is treated following the protocols for radio labeled material.
o Contaminated solid material (tips, tissues, etc) goes into the medibins
o Contaminated tubes are disposed in the medibins
o Take excess GMO material back to the VMT lab in secondary containment and destroy it there

Metabolic labeling of cells

- transport GGO’s in secondary containment to the isotope laboratory- in the isotope laboratory treat the GGO’s under the appropriate containment regime- grow cultures of max 5 mL in closed, plastic disposable tubes- distribute and spin down the cells in closed ependorf tubes in a tabletop centrifuge- separate pellet and supernatant. Chemically inactivate pellet. - process supernatant in disposables.- the following waste streams are discriminated:
o Inactivated, radiolabelled GMO’s are treated as radiolabeled waste.
o Radiolabeled supernatants are treated as radiolabelled waste.
o Contaminated solid material (tips, tissues, etc) goes into the medibins
o Contaminated culture tubes are disposed in the medibins
o Take excess GGO material back to the VMT lab in secondary containment and destroy it there
o decontaminate any set-up or work space that has been in contact with the GMO’s with 70% ethanol after use.