DNA polymerases




General use: use Phusion because of it's fidelity (and high yield) for proofreading and DyNAzyme II for other amplifications. Phusion can also be used for mutagenesis.

Extensor should only be used for really long amplicons. Extensor is not a proofreading enzyme and very expensive!!


Enzyme Fidelity 2x Mastermix Price (nov, 2008)
(compared to TAQ) per PCR (50 uL)
DyNAzyme II 1 Y 0.32
Pfu DNA polymerase 8 N 1.08
Extensor DNA polymerase 4 Y 3.05
Phusion DNA polymerase 52 Y 1.29




For further information ask Jeroen Nijland