General use: use Phusion because of it's fidelity (and high yield) for proofreading and DyNAzyme II for other amplifications. Phusion can also be used for mutagenesis.
Extensor should only be used for really long amplicons. Extensor is not a proofreading enzyme and very expensive!!
|Enzyme||Fidelity||2x Mastermix||Price (nov, 2008)|
|(compared to TAQ)||per PCR (50 uL)|
|Pfu DNA polymerase||8||N||1.08|
|Extensor DNA polymerase||4||Y||3.05|
|Phusion DNA polymerase||52||Y||1.29|
For further information ask Jeroen Nijland