Permission IG 05-049




This is an english summary of the official document 'Beschikking DGM/SAS IG 05-049' which is not yet available here



Title:

Heterologous and homologous expression of proteins in Sulfolobus


Section 1: mentioned in the text are

"Regeling" document describing legislation in relation to working with GMO's
"GGO" genetically modified organism
"appendix 1" list of non-pathogenic organisms
"appendix 2.1.1" list of vectors
"appendix 4" rules for working at ML-I and ML-II levels

The "Regeling" including the appendices can be viewed or downloaded in pdf format from the VROM site. Follow the link "Documenten" and then "Regelgeving". In the table select "Intergrale versie Regeling en Besluit oktober 2004". The latest update of appendix 2.1.1. can be viewed separately at the same site by selecting "Actuele vectorenlijst".


Section 2: allowed activities

2.1. Cloning in Escherichia coli

host Escherichia coli;
strains K12
vectors pMJ03
donor sequences genes coding for proteins or subunits of substrate transport systems protein translocation systems and derived mutants of these (Sulfolobus sp. and other hyperthermophilic archaea of class 1
containment level ML-I according to appendix 4.1.1.1

2.2. Construction of and activities with genetically modified Sulfolobus solfataricus

host Sulfolobus solfataricus;
vectors pMJ03
donor sequences genes coding for proteins or subunits of substrate transport systems protein translocation systems and derived mutants of these (Sulfolobus sp. and other hyperthermophilic archaea of class 1
containment level ML-II according to appendix 4.1.1.3

2.3. Activities are restricited to the Laboratory of Microbiology, University of Groningen, Kerklaan 30, Haren


Section 3: collaborators

All collaborators involved in the activities mentioned in section 2 must be informed about this permission and its contents before they can start their activities